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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
13/11/2015 |
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Actualizado : |
13/11/2015 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
LADO, J.; CRONJE, P.; ALQUÉZAR, B.; PAGE, A.; MANZI, M.; GÓMEZ-CADENAS, A.; STEAD, A.D.; ZACARÍAS, L.; RODRIGO, M.J. |
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Afiliación : |
JOANNA LADO LINDNER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Fruit shading enhances peel color, carotenes accumulation and chromoplast differentiation in red grapefruit. |
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Fecha de publicación : |
2015 |
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Fuente / Imprenta : |
Physiologia Plantarum, 2015, v.154, no. 4, p. 469-484. |
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Serie : |
0031-9317 |
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DOI : |
10.1111/ppl.12332 |
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Idioma : |
Inglés |
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Contenido : |
ABSTRACT.
The distinctive color of red grapefruits is due to lycopene, an unusual carotene in citrus. It has been observed that red ?Star Ruby? (SR) grapefruits grown inside the tree canopy develop a more intense red coloration than those exposed to higher light intensities. To investigate the effect of light on SR peel pigmentation, fruit were bagged or exposed to normal photoperiodic conditions, and changes in carotenoids, expression of carotenoid biosynthetic genes and plastid ultrastructure in the peel were analyzed. Light avoidance accelerated chlorophyll breakdown and induced carotenoid accumulation, rendering fruits with an intense coloration. Remarkably, lycopene levels in the peel of shaded fruits were 49-fold higher than in light-exposed fruit while concentrations of downstream metabolites were notably reduced, suggesting a bottleneck at the lycopene cyclization in the biosynthetic pathway. Paradoxically, this increment in carotenoids in covered fruit was not mirrored by changes in mRNA levels of carotenogenic genes, which were mostly up-regulated by light. In addition, covered fruits experienced profound changes in chromoplast differentiation, and the relative expression of genes related to chromoplast
development was enhanced. Ultrastructural analysis of plastids revealed an acceleration of chloroplasts to chromoplast transition in the peel of covered fruits concomitantly with development of lycopene crystals and plastoglobuli. In this sense, an accelerated differentiation of chromoplasts may provide biosynthetic capacity and a sink for carotenoids without involving major changes in transcript levels of carotenogenic genes. Light signals seem to regulate carotenoid accumulation at the molecular and structural level by
influencing both biosynthetic capacity and sink strength. Abbreviations ? 𝛽CHX, 𝛽-carotene hydroxylase; 𝛽LCY, lycopene cyclase 𝛽; ABA, abscisic acid; C, covered; Chl, chlorophyll; DXS, 1-deoxy-D-xylulose-5-phosphate synthase; FIB, fibrillin; FW, fresh weight; GGPP, geranyl geranyl pyrophosphate; GGPPS, geranyl geranyl pyrophosphate synthase; HDR, hydroxymethylbutenyl diphosphate reductase; HPLC, high-performance liquid chromatography; MEP, methyl-D-erythritol-4-phosphate; NC, non-covered; PCR, polymerase chain reaction; PDS, phytoene desaturase; PSY, phytoene synthase; sHSP, small heat shock protein; SR, Star Ruby; ZDS, 𝜁-carotene desaturase.
Physiol. Plant. MenosABSTRACT.
The distinctive color of red grapefruits is due to lycopene, an unusual carotene in citrus. It has been observed that red ?Star Ruby? (SR) grapefruits grown inside the tree canopy develop a more intense red coloration than those exposed to higher light intensities. To investigate the effect of light on SR peel pigmentation, fruit were bagged or exposed to normal photoperiodic conditions, and changes in carotenoids, expression of carotenoid biosynthetic genes and plastid ultrastructure in the peel were analyzed. Light avoidance accelerated chlorophyll breakdown and induced carotenoid accumulation, rendering fruits with an intense coloration. Remarkably, lycopene levels in the peel of shaded fruits were 49-fold higher than in light-exposed fruit while concentrations of downstream metabolites were notably reduced, suggesting a bottleneck at the lycopene cyclization in the biosynthetic pathway. Paradoxically, this increment in carotenoids in covered fruit was not mirrored by changes in mRNA levels of carotenogenic genes, which were mostly up-regulated by light. In addition, covered fruits experienced profound changes in chromoplast differentiation, and the relative expression of genes related to chromoplast
development was enhanced. Ultrastructural analysis of plastids revealed an acceleration of chloroplasts to chromoplast transition in the peel of covered fruits concomitantly with development of lycopene crystals and plastoglobuli. In this sense, an accelerated diff... Presentar Todo |
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Thesagro : |
CITRUS; CITRUS PARADISI. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 03223naa a2200265 a 4500
001 1053867
005 2015-11-13
008 2015 bl uuuu u00u1 u #d
024 7 $a10.1111/ppl.12332$2DOI
100 1 $aLADO, J.
245 $aFruit shading enhances peel color, carotenes accumulation and chromoplast differentiation in red grapefruit.$h[electronic resource]
260 $c2015
490 $a0031-9317
520 $aABSTRACT. The distinctive color of red grapefruits is due to lycopene, an unusual carotene in citrus. It has been observed that red ?Star Ruby? (SR) grapefruits grown inside the tree canopy develop a more intense red coloration than those exposed to higher light intensities. To investigate the effect of light on SR peel pigmentation, fruit were bagged or exposed to normal photoperiodic conditions, and changes in carotenoids, expression of carotenoid biosynthetic genes and plastid ultrastructure in the peel were analyzed. Light avoidance accelerated chlorophyll breakdown and induced carotenoid accumulation, rendering fruits with an intense coloration. Remarkably, lycopene levels in the peel of shaded fruits were 49-fold higher than in light-exposed fruit while concentrations of downstream metabolites were notably reduced, suggesting a bottleneck at the lycopene cyclization in the biosynthetic pathway. Paradoxically, this increment in carotenoids in covered fruit was not mirrored by changes in mRNA levels of carotenogenic genes, which were mostly up-regulated by light. In addition, covered fruits experienced profound changes in chromoplast differentiation, and the relative expression of genes related to chromoplast development was enhanced. Ultrastructural analysis of plastids revealed an acceleration of chloroplasts to chromoplast transition in the peel of covered fruits concomitantly with development of lycopene crystals and plastoglobuli. In this sense, an accelerated differentiation of chromoplasts may provide biosynthetic capacity and a sink for carotenoids without involving major changes in transcript levels of carotenogenic genes. Light signals seem to regulate carotenoid accumulation at the molecular and structural level by influencing both biosynthetic capacity and sink strength. Abbreviations ? 𝛽CHX, 𝛽-carotene hydroxylase; 𝛽LCY, lycopene cyclase 𝛽; ABA, abscisic acid; C, covered; Chl, chlorophyll; DXS, 1-deoxy-D-xylulose-5-phosphate synthase; FIB, fibrillin; FW, fresh weight; GGPP, geranyl geranyl pyrophosphate; GGPPS, geranyl geranyl pyrophosphate synthase; HDR, hydroxymethylbutenyl diphosphate reductase; HPLC, high-performance liquid chromatography; MEP, methyl-D-erythritol-4-phosphate; NC, non-covered; PCR, polymerase chain reaction; PDS, phytoene desaturase; PSY, phytoene synthase; sHSP, small heat shock protein; SR, Star Ruby; ZDS, 𝜁-carotene desaturase. Physiol. Plant.
650 $aCITRUS
650 $aCITRUS PARADISI
700 1 $aCRONJE, P.
700 1 $aALQUÉZAR, B.
700 1 $aPAGE, A.
700 1 $aMANZI, M.
700 1 $aGÓMEZ-CADENAS, A.
700 1 $aSTEAD, A.D.
700 1 $aZACARÍAS, L.
700 1 $aRODRIGO, M.J.
773 $tPhysiologia Plantarum, 2015$gv.154, no. 4, p. 469-484.
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Registro original : |
INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Tacuarembó. |
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Fecha actual : |
10/12/2015 |
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Actualizado : |
10/12/2015 |
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Tipo de producción científica : |
Abstracts/Resúmenes |
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Autor : |
DE BARBIERI, I.; CIAPPESONI, G.; RAMOS, Z.; MEDEROS, A.; PÉREZ JONES, J.; DONAGARAY, F.; FROS, A.; DUTRA, F.; GRATTAROLA, M.; GIMENO, D.; MONTOSSI, F. |
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Afiliación : |
LUIS IGNACIO DE BARBIERI ETCHEBERRY, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CARLOS GABRIEL CIAPPESONI SCARONE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ZULLY MARGOT RAMOS ALVEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; AMERICA ESTHER MEDEROS SILVEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; J. PÉREZ JONES, CRILU (Consorcio Regional de Innovación de Lanas Ultrafinas del Uruguay); F. DONAGARAY, SCMAU (Sociedad de Criadores Merino Autraliano del Uruguay); A. FROS, SCMAU (Sociedad de Criadores Merino Autraliano del Uruguay); F. DUTRA, CRILU (Consorcio Regional de Innovación de Lanas Ultrafinas del Uruguay); M. GRATTAROLA, SUL (Secretariado Uruguayo de la Lana).; D. GIMENO, SUL (Secretariado Uruguayo de la Lana).; FABIO MARCELO MONTOSSI PORCHILE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Innovaciones institucionales en el complejo textil-lanero del Uruguay: los casos del Proyecto Merino Fino y el Consorcio Regional de Innovación de Lanas Ultrafinas. |
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Fecha de publicación : |
2015 |
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Fuente / Imprenta : |
ln: Reunión ALPA (24., Puerto Varas, Chile). Resúmenes. Puerto Varas (Chile): ALPA, 2015. |
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Páginas : |
p. 764. |
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Idioma : |
Español |
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Palabras claves : |
DIÁMETRO DE LA FIBRA; PESO DE VELLÓN. |
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Thesagro : |
MEJORAMIENTO GENETICO; MERINO; OVINOS. |
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Asunto categoría : |
-- |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/5368/1/ALPA-2015-Resumen-Innovaciones.pdf
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Marc : |
LEADER 00930nam a2200289 a 4500
001 1054113
005 2015-12-10
008 2015 bl uuuu u01u1 u #d
100 1 $aDE BARBIERI, I.
245 $aInnovaciones institucionales en el complejo textil-lanero del Uruguay$blos casos del Proyecto Merino Fino y el Consorcio Regional de Innovación de Lanas Ultrafinas.$h[electronic resource]
260 $aln: Reunión ALPA (24., Puerto Varas, Chile). Resúmenes. Puerto Varas (Chile): ALPA$c2015
300 $ap. 764.
650 $aMEJORAMIENTO GENETICO
650 $aMERINO
650 $aOVINOS
653 $aDIÁMETRO DE LA FIBRA
653 $aPESO DE VELLÓN
700 1 $aCIAPPESONI, G.
700 1 $aRAMOS, Z.
700 1 $aMEDEROS, A.
700 1 $aPÉREZ JONES, J.
700 1 $aDONAGARAY, F.
700 1 $aFROS, A.
700 1 $aDUTRA, F.
700 1 $aGRATTAROLA, M.
700 1 $aGIMENO, D.
700 1 $aMONTOSSI, F.
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INIA Tacuarembó (TBO) |
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